Beilstein J. Nanotechnol.2012,3, 404–414, doi:10.3762/bjnano.3.47
silver nanoparticles are shown as an exemplary application for quantitative analysis.
Keywords: EGNP; enzymaticallygrownsilvernanoparticles; enzyme-induced deposition; nanoflower; SERS; Introduction
The application of metal nanoparticles in the field of bioanalytics extends the possibilities of
nanoparticles [19][20][21]. The characterization of such enzymaticallygrownsilvernanoparticles by atomic force microscopy [6] revealed an unusual, but very characteristic morphology: the desert-rose-like structure. This rose-like structure appears soon after the reaction is started and can be observed even
contaminations. Therefore, the enzymaticallygrownsilvernanoparticles were investigated by employing different microscopic techniques, namely scanning electron microscopy (SEM) and transmission electron microscopy (TEM), together with analytical methods, namely Rutherford backscattering spectrometry (RBS) and
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Figure 1:
Schematic sketch displaying the growth mechanism of the silver EGNP: (a) the substrates are immobil...